arc123 -NoNeed4aScreenName -arc123 -
No other docs taking over the reigns?
Still nobody dying. Sweden still there (and with lower fatalities YTD than 2018). PCR test still being used at 40x amplification.
It's not 40x it's an exponential amount of gamification. Its 40 cycles of amplification.
In theory you should be able to detect 1 copy of the virus in the cells with enough cycles. In practice that's not quite realistic but you can quantify viral RNA based and infer # of viruses present but it will still just he an estimate. An educated estimate
Genuine question - how would you compare your knowledge / experience in this field to Carl Heneghan (Head of Evidence Based medicine at Oxford Uni).
im not going to be deceitful - I am expert in some areas, but this is not one of them. I am heavily influenced in my opinions by him and a building number if similar view points. I cannot debate with you directly so I’d like it if you could help me with understanding the differences in these view points, as they obviously cannot both be correct.
I think you could be misrepresenting what he is saying. I havent read it but PCR works in cycles. Every cycle your target doubles (in theory) so if you have 1 copy after a second cycle you have 2, then 4, then 8, then 16..etc.
The CT value is only when the signal starts to go into exponential phase and rises above background noise.
You also need to understand though in these experiments they are using very low starting material with viral RNA.
When you amplify your target you need to create primers. These primers will flank your target sequence or the gene you are interested in.
For COVID its novel so theres something different from all other coronviruses. The primers should flank this novel sequence which, in theory, would then exclude all other viruses except Covid 19
Is it making sense how I am explaining it?